Brenda Grizzle
2180 E. Galbraith Rd
Building F Room 217
Cincinnati, Ohio 45237
Office (513)558-0365
Zebrafish Core (513)558-0490
Zebrafish Lab (513)558-2319
Cell (513)382-5821

Email Brenda

Works in the Hove Zebrafish laboratory

Responsible for all aspects of maintenance and husbandry of Zebrafish and
Medaka in the Genome Research Institute’s fish facility. Other
Responsibilities include: Ordering equipment and supplies for core facility
Obtaining new fish strains from and shipping available strains to laboratories around the world
Maintaining LAMS and IACUC compliance of core facility
Preparation of microbiological (e.g. Paramecium sp), phytoplankton (e.g. rotifers), and Artemia cultures
Feeding, anesthesia, and euthanasia of larval and adult fish
Evaluating efficacy of altered dietary regimes on fish health and reproduction
Monitoring water quality including temperature, ammonia,
nitrite, nitrate, pH, conductivity, hardness, and salinity.
Assessing and changing mechanical, biological and chemical filtering systems.
Setting - up breeder and embryo delivery for PIs at GRI
Collecting, cleaning, sterilizing, and dechorionating eggs for research and stocking
Developing and improving core standard operating procedures
Maintaining and improving quarantine and sentinel protocols
Assessing fish health and interpreting pathology findings
Ensuring sterilization of glassware, tanks, and appurtenances